Femtosecond biophotonics : core technology and applications / by Min Gu [and others].

Includes bibliographical references and index.

Print version record.

Cover; Half-title; Title; Copyright; Dedication; Contents; Preface; 1 Introduction; 1.1 Femtosecond biophotonics; 1.2 Scope of the book; References; 2 Nonlinear optical microscopy; 2.1 Nonlinear optical microscopy; 2.1.1 Multi-photon fluorescence microscopy; 2.1.2 Harmonic generation microscopy; 2.1.3 Coherent anti-Stokes Raman scattering microscopy; 2.2 Two-photon fluorescence and second harmonic generation microscopy; 2.2.1 Comparison of single-photon and two-photon fluorescence imaging; 2.2.2 Reflection second harmonic generation microscopy through tissue.

2.3 Three-dimensional two-photon autofluorescence spectroscopy2.4 Effect of handling and fixation processes on two-photon autofluorescence spectroscopy; 2.5 Two-photon excitation fluorescence resonance energy transfer; 2.6 Two-photon fluorescence lifetime imaging; References; 3 Two-photon fluorescence microscopy through turbid media; 3.1 Two-photon fluorescence microscopy of microspheres embedded in turbid media; 3.1.1 Measurement of two-photon fluorescence images; 3.1.2 Comparison with Monte-Carlo simulation; 3.2 Limiting factors on image quality in imaging through turbid media.

3.3 Quantitative comparison of penetration depth between two-photon excitation and single-photon excitationReferences; 4 Fibre-optical nonlinear microscopy; 4.1 Fibre-optical confocal microscopy; 4.1.1 Image formation; 4.1.2 Milestones in fibre-optical confocal microscopy; 4.2 Two-photon fluorescence imaging systems using a single-mode optical fibre coupler; 4.2.1 Fibre-optical two-photon fluorescence microscopy; 4.2.2 Coupling efficiency and splitting ratio; 4.2.3 Spectral and temporal broadening; 4.2.4 Fluorescence axial response; 4.2.5 Three-dimensional optical transfer function analysis.

4.2.6 Discussion4.3 Fibre-optical second harmonic generation microscopy; 4.3.1 Coupling efficiency and splitting ratio; 4.3.2 Second-harmonic generated axial response; 4.3.3 Three-dimensional coherent transfer function analysis; 4.3.4 Polarisation anisotropy; 4.4 Towards nonlinear endoscopic imaging; 4.5 Summary; References; 5 Nonlinear optical endoscopy; 5.1 An introduction to nonlinear optical endoscopy; 5.1.1 Optical fibres and ultrashort pulse delivery; 5.1.2 Scanning mechanisms; 5.1.3 Geometries of fibre-optical nonlinear optical microscopy.

5.2 Nonlinear optical microscopy using double-clad PCFs5.2.1 Characterisation of double-clad PCFs; 5.2.2 Experimental arrangement; 5.2.3 Axial resolution; 5.2.4 Improvement of signal level; 5.2.5 Nonlinear optical imaging; 5.2.6 SHG polarisation anisotropy measurement; 5.3 A nonlinear optical endoscope based on a double-clad PCF and a MEMS mirror; 5.3.1 Endoscope design; 5.3.2 Axial resolution and signal level; 5.3.3 Endoscopic imaging; 5.3.4 3D tissue imaging; 5.4 Nonlinear optical microscopy using a double-clad PCF coupler; 5.4.1 A double-clad PCF coupler; 5.4.2 Experimental arrangement.

Covering key techniques for optical microscopy and micro-fabrication, this book provides the first detailed treatment of femtosecond laser-based biophotonics.

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