Femtosecond biophotonics : core technology and applications /
Femtosecond biophotonics : core technology and applications /
by Min Gu [and others].
- Cambridge ; New York : Cambridge University Press, 2010.
- 1 online resource (xii, 232 pages) : illustrations
Includes bibliographical references and index.
Cover; Half-title; Title; Copyright; Dedication; Contents; Preface; 1 Introduction; 1.1 Femtosecond biophotonics; 1.2 Scope of the book; References; 2 Nonlinear optical microscopy; 2.1 Nonlinear optical microscopy; 2.1.1 Multi-photon fluorescence microscopy; 2.1.2 Harmonic generation microscopy; 2.1.3 Coherent anti-Stokes Raman scattering microscopy; 2.2 Two-photon fluorescence and second harmonic generation microscopy; 2.2.1 Comparison of single-photon and two-photon fluorescence imaging; 2.2.2 Reflection second harmonic generation microscopy through tissue. 2.3 Three-dimensional two-photon autofluorescence spectroscopy2.4 Effect of handling and fixation processes on two-photon autofluorescence spectroscopy; 2.5 Two-photon excitation fluorescence resonance energy transfer; 2.6 Two-photon fluorescence lifetime imaging; References; 3 Two-photon fluorescence microscopy through turbid media; 3.1 Two-photon fluorescence microscopy of microspheres embedded in turbid media; 3.1.1 Measurement of two-photon fluorescence images; 3.1.2 Comparison with Monte-Carlo simulation; 3.2 Limiting factors on image quality in imaging through turbid media. 3.3 Quantitative comparison of penetration depth between two-photon excitation and single-photon excitationReferences; 4 Fibre-optical nonlinear microscopy; 4.1 Fibre-optical confocal microscopy; 4.1.1 Image formation; 4.1.2 Milestones in fibre-optical confocal microscopy; 4.2 Two-photon fluorescence imaging systems using a single-mode optical fibre coupler; 4.2.1 Fibre-optical two-photon fluorescence microscopy; 4.2.2 Coupling efficiency and splitting ratio; 4.2.3 Spectral and temporal broadening; 4.2.4 Fluorescence axial response; 4.2.5 Three-dimensional optical transfer function analysis. 4.2.6 Discussion4.3 Fibre-optical second harmonic generation microscopy; 4.3.1 Coupling efficiency and splitting ratio; 4.3.2 Second-harmonic generated axial response; 4.3.3 Three-dimensional coherent transfer function analysis; 4.3.4 Polarisation anisotropy; 4.4 Towards nonlinear endoscopic imaging; 4.5 Summary; References; 5 Nonlinear optical endoscopy; 5.1 An introduction to nonlinear optical endoscopy; 5.1.1 Optical fibres and ultrashort pulse delivery; 5.1.2 Scanning mechanisms; 5.1.3 Geometries of fibre-optical nonlinear optical microscopy. 5.2 Nonlinear optical microscopy using double-clad PCFs5.2.1 Characterisation of double-clad PCFs; 5.2.2 Experimental arrangement; 5.2.3 Axial resolution; 5.2.4 Improvement of signal level; 5.2.5 Nonlinear optical imaging; 5.2.6 SHG polarisation anisotropy measurement; 5.3 A nonlinear optical endoscope based on a double-clad PCF and a MEMS mirror; 5.3.1 Endoscope design; 5.3.2 Axial resolution and signal level; 5.3.3 Endoscopic imaging; 5.3.4 3D tissue imaging; 5.4 Nonlinear optical microscopy using a double-clad PCF coupler; 5.4.1 A double-clad PCF coupler; 5.4.2 Experimental arrangement.
Covering key techniques for optical microscopy and micro-fabrication, this book provides the first detailed treatment of femtosecond laser-based biophotonics.
9780511729713 (electronic bk.) 0511729715 (electronic bk.) 0511726422 (electronic bk.) 9780511726422 (electronic bk.) 9780511730313 (electronic bk.) 0511730314 (electronic bk.) 9780511727818 051172781X
9786612619953
261995 MIL
GBB027857 bnb
101567614 DNLM 015489420 Uk
Femtosecond lasers.
Photonics.
Photobiology.
Confocal microscopy.
Endoscopy.
Multiphoton excitation microscopy.
Nonlinear optical microscopy.
Microscopy, Confocal
Endoscopy
Microscopy, Fluorescence, Multiphoton
Optical Tweezers
Photobiology
Nonlinear Optical Microscopy
Lasers femtosecondes.
Photonique.
Photobiologie.
Microscopie confocale.
Endoscopie.
Microscopie optique non linéaire.
TECHNOLOGY & ENGINEERING--Lasers & Photonics.
Femtosecond lasers.
Photobiology.
Photonics.
Bildgebendes Verfahren
Biophotonik
Mikroskopie
Molekularbiologie
Electronic books.
QH324.9.L37 / F45 2010eb
621.366
2011 J-891 QH 324.9.L37
Includes bibliographical references and index.
Cover; Half-title; Title; Copyright; Dedication; Contents; Preface; 1 Introduction; 1.1 Femtosecond biophotonics; 1.2 Scope of the book; References; 2 Nonlinear optical microscopy; 2.1 Nonlinear optical microscopy; 2.1.1 Multi-photon fluorescence microscopy; 2.1.2 Harmonic generation microscopy; 2.1.3 Coherent anti-Stokes Raman scattering microscopy; 2.2 Two-photon fluorescence and second harmonic generation microscopy; 2.2.1 Comparison of single-photon and two-photon fluorescence imaging; 2.2.2 Reflection second harmonic generation microscopy through tissue. 2.3 Three-dimensional two-photon autofluorescence spectroscopy2.4 Effect of handling and fixation processes on two-photon autofluorescence spectroscopy; 2.5 Two-photon excitation fluorescence resonance energy transfer; 2.6 Two-photon fluorescence lifetime imaging; References; 3 Two-photon fluorescence microscopy through turbid media; 3.1 Two-photon fluorescence microscopy of microspheres embedded in turbid media; 3.1.1 Measurement of two-photon fluorescence images; 3.1.2 Comparison with Monte-Carlo simulation; 3.2 Limiting factors on image quality in imaging through turbid media. 3.3 Quantitative comparison of penetration depth between two-photon excitation and single-photon excitationReferences; 4 Fibre-optical nonlinear microscopy; 4.1 Fibre-optical confocal microscopy; 4.1.1 Image formation; 4.1.2 Milestones in fibre-optical confocal microscopy; 4.2 Two-photon fluorescence imaging systems using a single-mode optical fibre coupler; 4.2.1 Fibre-optical two-photon fluorescence microscopy; 4.2.2 Coupling efficiency and splitting ratio; 4.2.3 Spectral and temporal broadening; 4.2.4 Fluorescence axial response; 4.2.5 Three-dimensional optical transfer function analysis. 4.2.6 Discussion4.3 Fibre-optical second harmonic generation microscopy; 4.3.1 Coupling efficiency and splitting ratio; 4.3.2 Second-harmonic generated axial response; 4.3.3 Three-dimensional coherent transfer function analysis; 4.3.4 Polarisation anisotropy; 4.4 Towards nonlinear endoscopic imaging; 4.5 Summary; References; 5 Nonlinear optical endoscopy; 5.1 An introduction to nonlinear optical endoscopy; 5.1.1 Optical fibres and ultrashort pulse delivery; 5.1.2 Scanning mechanisms; 5.1.3 Geometries of fibre-optical nonlinear optical microscopy. 5.2 Nonlinear optical microscopy using double-clad PCFs5.2.1 Characterisation of double-clad PCFs; 5.2.2 Experimental arrangement; 5.2.3 Axial resolution; 5.2.4 Improvement of signal level; 5.2.5 Nonlinear optical imaging; 5.2.6 SHG polarisation anisotropy measurement; 5.3 A nonlinear optical endoscope based on a double-clad PCF and a MEMS mirror; 5.3.1 Endoscope design; 5.3.2 Axial resolution and signal level; 5.3.3 Endoscopic imaging; 5.3.4 3D tissue imaging; 5.4 Nonlinear optical microscopy using a double-clad PCF coupler; 5.4.1 A double-clad PCF coupler; 5.4.2 Experimental arrangement.
Covering key techniques for optical microscopy and micro-fabrication, this book provides the first detailed treatment of femtosecond laser-based biophotonics.
9780511729713 (electronic bk.) 0511729715 (electronic bk.) 0511726422 (electronic bk.) 9780511726422 (electronic bk.) 9780511730313 (electronic bk.) 0511730314 (electronic bk.) 9780511727818 051172781X
9786612619953
261995 MIL
GBB027857 bnb
101567614 DNLM 015489420 Uk
Femtosecond lasers.
Photonics.
Photobiology.
Confocal microscopy.
Endoscopy.
Multiphoton excitation microscopy.
Nonlinear optical microscopy.
Microscopy, Confocal
Endoscopy
Microscopy, Fluorescence, Multiphoton
Optical Tweezers
Photobiology
Nonlinear Optical Microscopy
Lasers femtosecondes.
Photonique.
Photobiologie.
Microscopie confocale.
Endoscopie.
Microscopie optique non linéaire.
TECHNOLOGY & ENGINEERING--Lasers & Photonics.
Femtosecond lasers.
Photobiology.
Photonics.
Bildgebendes Verfahren
Biophotonik
Mikroskopie
Molekularbiologie
Electronic books.
QH324.9.L37 / F45 2010eb
621.366
2011 J-891 QH 324.9.L37