000			08040cam a2200937Ma 4500
001			ocn314216993
003			OCoLC
005			20220713001213.0
006			m o d
007			cr zn|||||||||
008			040402s2004 enka ob 001 0 eng d
010			_a 2004299783
040			_aCN8ML _beng _epn _cCN8ML _dOCLCQ _dN$T _dE7B _dOCLCQ _dIDEBK _dMERUC _dOCLCQ _dGZM _dOCLCO _dOCLCF _dOCLCQ _dNLGGC _dYDXCP _dEBLCP _dMHW _dDEBSZ _dOCLCQ _dTOA _dAGLDB _dOTZ _dOCLCO _dOCLCQ _dOCLCA _dOCLCQ _dOCLCO _dOCLCQ _dSTF _dOCLCO _dVNS _dOCLCO _dVTS _dOCLCO _dCEF _dCOCUF _dAU@ _dOCLCO _dLHU _dFVL _dOCLCQ _dOCLCA _dS9I _dYOU _dCANPU _dREC _dCNTRU _dMT4IT _dLOA _dK6U _dVT2 _dOCLCO _dOCLCQ _dSFB _dOCLCA _dAJS _dOCLCO
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020			_a9780191516450 _q(electronic bk.)
020			_a0191516457 _q(electronic bk.)
020			_a9786610845293
020			_a6610845298
020			_a9780199638734
020			_a019963873X
020			_z019963873X _q(Paper)
020			_z0199638748 _q(Cloth)
020			_z9780199638741
027			_aMYILIB_CUp
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029	1		_aDEBBG _bBV043126782
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035			_a(OCoLC)314216993 _z(OCoLC)316043706 _z(OCoLC)455959813 _z(OCoLC)476259877 _z(OCoLC)646784754 _z(OCoLC)756879021 _z(OCoLC)994691172 _z(OCoLC)1035659532 _z(OCoLC)1044148989 _z(OCoLC)1044294206 _z(OCoLC)1056326933 _z(OCoLC)1056353918 _z(OCoLC)1059562508 _z(OCoLC)1059786311 _z(OCoLC)1077822793 _z(OCoLC)1097260816 _z(OCoLC)1099557162 _z(OCoLC)1107715826 _z(OCoLC)1109953112 _z(OCoLC)1111283265 _z(OCoLC)1117840156 _z(OCoLC)1125449263 _z(OCoLC)1132017190 _z(OCoLC)1136452650
037			_a084529 _bMIL
050		4	_aQR342 _b.P43 2004eb
055	1	3	_aQR342 _b.P43 2004eb
060		4	_a2004 F-484
060		4	_aQU 68 _bP532 2004
070			_aQR342 _b.P43 2004
072		7	_aSCI _x008000 _2bisacsh
072		7	_aSCI _x045000 _2bisacsh
082	0	4	_a579.2/6 _222
049			_aMAIN
245	0	0	_aPhage display : _ba practical approach / _cedited by Tim Clackson, Henry B. Lowman.
260			_aOxford ; _aNew York : _bOxford University Press, _c©2004.
300			_a1 online resource (xxiv, 332 pages) : _billustrations
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_adata file _2rda
490	1		_aPractical approach series ; _vno. 266
504			_aIncludes bibliographical references and index.
588	0		_aPrint version record.
505	0		_aCover -- Contents -- Protocol list -- List of abbreviations -- List of contributors -- 1 Introduction to phage biology and phage display -- 1 Introduction -- 2 Biology of filamentous phage -- 2.1 Introduction -- 2.2 Structure of the phage particle -- 2.3 Infection -- 2.4 Replication -- 2.5 Genes and gene expression -- 2.6 Physiology of phage assembly -- 2.7 The mechanics of phage assembly -- 3 Coat proteins used for display -- 3.1 pVIII -- 3.2 pIll -- 4 Starting a phage display project -- 4.1 Feasibility of display -- 4.2 Phage or phagemid vector? -- 4.3 Polyvalent or monovalent display? -- 4.4 Helper phage -- 4.5 General protocols for phage preparation and quantitation -- 5 General principles of a phage display project -- 5.1 Making a library -- 5.2 Selection -- 5.3 Analysis of clones -- 6 Common problems -- 6.1 Library quality -- 6.2 Expression editing -- 6.3 Over-selection -- 7 Alternative display systems -- 8 Commercial sources of phage display libraries and kits -- References -- 2 Constructing phage display libraries by oligonucleotide-directed mutagenesis -- 1 Introduction -- 2 Considerations for library design -- 2.1 Site-directed mutagenesis -- 2.2 Degenerate codon design -- 2.3 Theoretical versus actual diversity -- 3 Oligonucleotide-directed mutagenesis -- 3.1 Oligonucleotide-directed mutagenesis versus cassette mutagenesis -- 3.2 The chemistry and biology of oligonucleotide-directed mutagenesis -- 3.3 Construction of an inactive template -- 4 Library construction and storage -- 4.1 Preparation of single-stranded DNA template -- 4.2 In vitro synthesis of heteroduplex CCC-dsDNA -- 4.3 E.coli electroporation and production of library phage -- 4.4 Library storage and reinfection -- 5 Biological reagents -- References -- 3 In vitro DNA recombination -- 1 Introduction -- 2 Background to in vitro DNA recombination -- 2.1 Use of in vitro DNA recombination in directed evolution -- 2.2 Applications of in vitro DNA recombination -- 2.3 Recombination statistics -- 3 Methods for in vitro DNA recombination -- 3.1 Stemmer method -- 3.2 Random DNA fragmentation with endonuclease V from E. coli -- 3.3 Random priming recombination -- 3.4 Staggered Extension Process (StEP) -- 3.5 In vitro heteroduplex formation and in vivo repair (heteroduplex recombination) -- 3.6 Choice of recombination method -- 3.7 Removal of background -- 3.8 Technical tips -- References -- 4 Phage selection strategies for improved affinity and specificity of proteins and peptides -- 1 Introduction -- 2 Vector considerations -- 2.1 Monovalent and polyvalent phage display -- 2.2 Confirming display -- 2.3 Protein expression from phagemid vectors -- 2.4 Vector construction and phagemid preparation -- 3 Library design -- 3.1 Hard randomization -- 3.2 Soft randomization -- 4 Target presentation -- 4.1 Direct immobilization -- 4.2 Solution binding -- 4.3 Blocking -- 4.4 Pilot selection -- 5 Selection -- 5.1 Binding buffer considerations -- 5.2 Stringency of selection -- 5.3 Competitive selection -- 5.4 Elution of bound phage -- 5.5 Amplification -- 5.6 Monitori.
520			_aThis new book aims to enable researchers to design and undertake all aspects of a phage display project, from designing an experimental strategy and constructing a library to performing selections and analyzing the results. All of the protocols and chapters are extensively cross-referenced, allowing readers to move beyond the specific examples provided in order to customize the procedures for their own protein or selection system of interest. Phage Display is an up-to-date, . comprehensive and integrated experimental guide to the technique, which is essential reading for anyone currently using.
590			_aeBooks on EBSCOhost _bEBSCO eBook Subscription Academic Collection - Worldwide
650		0	_aBacteriophages. _9205798
650		0	_aViral proteins. _9205799
650		0	_aMicrobial biotechnology. _9188669
650		2	_aPeptide Library _91427276
650		2	_aViral Proteins _9205799
650		2	_aBacteriophages _9205798
650		6	_aBactériophages. _9933376
650		6	_aProtéines virales. _91383375
650		6	_aBiotechnologie microbienne. _9920488
650		7	_aSCIENCE _xLife Sciences _xBiology. _2bisacsh _9864707
650		7	_aSCIENCE _xLife Sciences _xMicrobiology. _2bisacsh _9864708
650		7	_aBacteriophages. _2fast _0(OCoLC)fst00825287 _9205798
650		7	_aMicrobial biotechnology. _2fast _0(OCoLC)fst01019471 _9188669
650		7	_aViral proteins. _2fast _0(OCoLC)fst01167620 _9205799
655		0	_aElectronic books.
655		4	_aElectronic books.
700	1		_aClackson, Tim. _9205800
700	1		_aLowman, Henry B. _9205801
776	0	8	_iPrint version: _tPhage display. _dOxford ; New York : Oxford University Press, ©2004 _z0199638748 _w(DLC) 2004299783 _w(OCoLC)54904081
830		0	_aPractical approach series ; _v266. _9205802
856	4	0	_uhttps://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=264953
938			_aEBL - Ebook Library _bEBLB _nEBL422829
938			_aebrary _bEBRY _nebr10266414
938			_aEBSCOhost _bEBSC _n264953
938			_aProQuest MyiLibrary Digital eBook Collection _bIDEB _n84529
938			_aYBP Library Services _bYANK _n2952200
994			_a92 _bINOPJ
999			_c2907727 _d2907727