Flow cytometry : principles, methodology and applications / Stefanos Papandreou, editor.

Includes bibliographical references and index.

Description based on print version record.

FLOW CYTOMETRY. PRINCIPLES, METHODOLOGY AND APPLICATIONS -- FLOW CYTOMETRY. PRINCIPLES, METHODOLOGY AND APPLICATIONS -- Library of Congress Cataloging-in-Publication Data -- Contents -- Preface -- Chapter 1: A Few Tests Prior to Flow Cytometry and Epifluorescence Analyses of Freshwater Bacterio -And Virioplankton Communities -- Abstract -- Introduction -- Materials and Methods -- Results -- Discussion -- Conclusion -- Acknowledgment -- References

Chapter 2: Application of Flow Cytometry in Phenotyping, Analysis and Functional Characterization of T Regulatory and Immune Suppressor Cells: A Focus on Multiple Myeloma Immune Regulatory BiologyAbstract -- Introduction -- Ontogeny of T Regulatory Cells -- FoxP3 Gene and Their Role in T Regulatory Cell Differentiation -- Role of FoxP3 in T Regulatory Cells -- Suppressive Mechanisms of T Regulatory Cells -- Delineation of Phenotypic Features of CD4 T Regulatory Cells -- Subsets of CD4 T Regulatory Cells

""Assessment of Suppressive Function of T Regulatory Cells: Flow Cytometry Versus Thymidine Uptake Method""""CD8 T Regulatory Cells: A Novel Suppressor""; ""Suppressive Mechanisms of CD8 Treg Cell Subsets""; ""Myeloid-Derived Suppressor Cells""; ""Immune Regulatory Biology of Multiple Myeloma""; ""Conclusion""; ""Acknowledgment""; ""References""; ""Chapter 3: Multicolor Panel Design Strategies in Rare Event Analysis: Dendritic Cells and Regulatory T Cells""; ""Abstract""; ""1. Introduction""; ""2. How to Avoid Background (“Noise�)""; ""3. Reagent Selection""

4. Limiting Spectral Overlap5. Technical Limitations of Tandem Dyes -- 6. Appropriate Controls to Validate Your Experiment -- 7. How Many Events Must be Processed? -- 8. Multicolor Panel Design to Detect Rare Events: Two Examples -- Conclusion -- References -- Chapter 4: Light-Scattering Flow Cytometry: Advanced Characterization of Individual Particle Morphology -- Abstract -- Introduction -- Instrument -- Application -- Conclusion -- Acknowledgments -- References -- Chapter 5: Applications of Flow Cytometry in Environmental Sciences: Inspiring Examples

AbstractIntroduction -- Microbial Pollution of Drinking Water -- Viability of Bees Spermatozoa -- Monitoring the Cyanobacteria -- Conclusion -- References -- Index

Flow cytometry is a method to conduct a multiparameter analysis of cells suspended in liquid and passing through a laser beam. As cells pass through the flow chamber they are hit with the laser light beam which is scattered in different directions and recorded as forward light scatter and side light scatter. In this book, the authors discuss the principles, methodology and applications of flow cytometry. Topics include flow cytometry and epifluorescence analyses of freshwater bacterio- and virioplankton communities; application of flow cytometry in phenotyping, analysis and functional characte.

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