Methodologies for metabolomics : experimental strategies and techniques / edited by Norbert W. Lutz, Jonathan V. Sweedler, Ron A. Wevers.

"While most of the focus in "omics" science over the past decade has been on sequencing the human genome [1] or annotating the human proteome [2], there is another equally important component of the human body that has, until recently, been largely overlooked: the human metabolome. The human metabolome can be thought of as the complete collection of small molecule metabolites found in our bodies. These small molecules include such chemical entities as peptides, amino acids, nucleic acids, carbohydrates, organic acids, vitamins, minerals, food additives, drugs and just about any other chemical (with a molecular weight 1500 Da) that can be used, ingested or synthesized by humans. Metabolites act as the bricks and mortar of our cells. They serve as the building blocks for all of our macromolecules including proteins, RNA, DNA, carbohydrates, membranes and all other biopolymers that give our cells their structure and integrity. Metabolites also act as the fuel for all cellular processes, the buffers to help tolerate environmental insults and the messengers for most intra- and intercellular events. Together with the genome and the proteome, the human metabolome essentially defines who and what we are."-- Provided by publisher.

Section 1: Basic Methodological Strategies in Metabolomic Research -- Exploring the Human Metabolome by Nuclear Magnetic Resonance Spectroscopy and Mass Spectrometry -- Methodological Requirements for Lipidomics Research -- Biological Methods for Metabolic Research -- Section 2: Metabolomic Mass Spectrometry: Experimental Techniques and Bioinformatics -- Considerations in Sample Preparation, Collection, and Extraction Approaches Applied in Microbial, Plant, and Mammalian Metabolic Profiling -- Mass Spectrometry-Based Methodologies for Single-Cell Metabolite Detection and Identification -- Direct Metabolomics from Tissues and Cells: Laser Ablation Electrospray Ionization for Small Molecule and Lipid Characterization -- Bioinformatic Approaches to Processing and Annotation of High-Resolution Mass Spectrometry Data -- Approaches for Natural Product Detection and Structural Elucidation Using Mass Spectrometry with High Mass Accuracy -- Metabolomics Using Ion Mobility Mass Spectrometry -- Metabolmics via Biomedical Mass Spectrometry: From Sampling to Clinical Applications -- Section 3: Metabolomics of Biofluids: Nuclear Magnetic Resonance Spectroscopy and Chemometrics -- Analytical Techniques in Metabolomics Integrating Nuclear Magnetic Resonance Spectroscopy and Chromatography with Mass Spectrometry -- Chemometric Methods in Nuclear Magnetic Resonance-Based Body Fluid Analysis -- Nuclear Magnetic Resonance of Cerebrospinal Fluid: The Neurometabolome -- Nuclear Magnetic Resonance-Based Saliva Metabolomics -- Nuclear Magnetic Resonance Methods for Metabolomic Investigation of Amniotic Fluid -- Nuclear Magnetic Resonance Analysis and Genetic Metabolic Disease -- Lipid Profiling in Health and Disease -- Section 4: Metabolomic Nuclear Magnetic Resonance Spectroscopy Techniques for Body Tissue Analysis -- Magnetic Resonance Spectroscopy in Investigating the Cancer Metabolome in Preclinical Model Systems -- Phospholipidomics by Phosphorus Nuclear Magnetic Reasonance Spectroscopy of Tissue Extracts -- Carbon-13 Nuclear Magnetic Resonance for Analysis of Metabolic Pathways -- Hyperpolarized Nuclear Magnetic Resonance Spectroscopy: A New Method for Metabolomic Research -- Metabolomic Magnetic Reasonance Spectroscopy of Human Tissues: Comparison of In Vivo and High-Resolution Magic Angle Spinning Ex Vivo Techniques -- Reproducible Sample Preparation and Spectrum Acquisition Techniques for Metabolic Profiling of Human Tissues by Proton High-Resolution Magic Angle Spinning Nuclear Magnetic Resonance -- Assignment Strategies for Nuclear Magnetic Resonances in Metabolomic Research

Print version record.

Includes bibliographical references and index.

English.

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