Embryonic stem cells : a practical approach / edited by Elena Notarianni, Martin J. Evans.

Includes bibliographical references and index.

Procedures for deriving ES cell lines from the mouse / Frances A. Brook -- Production of ES cell-derived mice / Yoko Kato and Yukio Tsunoda -- Generating animal models of human mitochondrial genetic disease using mouse ES cells / Grant R. MacGregor [and others] -- Controlling the differentiation of mouse ES cells in vitro / Michael V. Wiles and Gabriele Proetzel -- In vitro differentiation of mouse ES cells into muscle cells / Yelena S. Tarasova [and others] -- In vitro differentiation of mouse ES cells into haematopoietic cells / Osamu Ohneda and Masayuki Yamamoto -- Lineage selection and transplantation of mouse ES cell-derived neural precursors / Tanja Schmandt, Tamara Glaser and Oliver Brüstle -- In vitro differentiation of mouse ES cells into pancreatic and hepatic cells / Przemyslaw Blyszczuk, Gabriela Kania and Anna M. Wobus -- Isolation and characterization of human ES cells / Martin F. Pera -- Differentiation of human ES cells / Sharon Gerecht-Nir and Joseph Itskovitz-Eldor -- ES cell lines from the cynomolgus monkey (Macaca fascicularis) / Hirofumi Suemori [and others].

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This book serves as a primer to ES cells, their derivation and experimental manipulation. It contains a broad compendium of methods of direct relevance to both graduate students and specialist researchers. An introductory chapter by the principle originator of ES cell research outlines the fundamentals and charts the development of the field. This is followed by comprehensive coverage of state-of-the art techniques for ES cell manipulation, with the mouse as the experimental paradigm, and by recent innovations with ES cells from human and non-human primates. ES cell-based therapies for otherwise intractable diseases are now being developed with the present challenge to control ES cell growth and differentiation for applications such as cell transplantation - a recurrent theme in this book. As a volume in the Practical Approach Series, the emphasis is on current methods from recognised experts.

English.

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