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Biomedical electron microscopy : illustrated methods and interpretations / Arvid B. Maunsbach, Björn A. Afzelius.

By: Contributor(s): Material type: TextTextPublication details: San Diego, Calif. : Academic Press, ©1999.Description: 1 online resource (xv, 548 pages) : illustrations (some color)Content type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 9780080528090
  • 0080528090
  • 128127948X
  • 9781281279484
  • 9786611279486
  • 6611279482
Subject(s): Genre/Form: Additional physical formats: Print version:: Biomedical electron microscopy.DDC classification:
  • 570/.28/25 22
LOC classification:
  • QH212.E4 M38 1999eb
NLM classification:
  • 1999 A-682
  • QY 17
Online resources:
Contents:
Preface. Acknowledgments. Micrograph Interpretation. Fixatives. Fixative Vehicle. Fixative Application. Dehydration and Embedding. Freezing and Freeze-Substitution. Support Films. Ultramicrotomy. Section-Staining. Microscopy. Image Recording. Photographic and Digital Printing. Negative Staining. Autoradiography. Cytochemistry. Immunocytochemistry. Freeze-Fracturing and Shadowing. Sampling and Quantitation. Image Processing. 3D Reconstructions. Appendix: Practical Methods. Index. Acknowledgment for Figures.
Summary: This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis. Key Features * Authored by the key leaders in the biological electron microscopy field * Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines * Introduces students on how to read and interpret electron micrographs.
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Electronic-Books Electronic-Books OPJGU Sonepat- Campus E-Books EBSCO Available

Includes bibliographical references and index.

Print version record.

Preface. Acknowledgments. Micrograph Interpretation. Fixatives. Fixative Vehicle. Fixative Application. Dehydration and Embedding. Freezing and Freeze-Substitution. Support Films. Ultramicrotomy. Section-Staining. Microscopy. Image Recording. Photographic and Digital Printing. Negative Staining. Autoradiography. Cytochemistry. Immunocytochemistry. Freeze-Fracturing and Shadowing. Sampling and Quantitation. Image Processing. 3D Reconstructions. Appendix: Practical Methods. Index. Acknowledgment for Figures.

This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis. Key Features * Authored by the key leaders in the biological electron microscopy field * Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines * Introduces students on how to read and interpret electron micrographs.

English.

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