Molecular methods for virus detection / edited by Danny L. Wiedbrauk, Daniel H. Farkas.

Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. Key Features * Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions * Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT * Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence * Includes quality control information crucial in research and clinical laboratories * Most chapters are written by the inventors and principal developers of the methodologies * Includes color plates, 77 figures, and 18 tables.

D.L. Wiedbrauk and A.M. Drevon, Nucleic Acid Detection Methods. -- D.L. Wiedbrauk and J. Stoerker, Quality Assurance in the Molecular Biology Laboratory. -- D.L. Stoler and N.L. Michael, Nucleic Acid Blotting Techniques for Virus Detection. -- J. Carr, In Situ Hybridization. -- R.L. Hodinka, Antiviral Susceptibility Testing Using DNA-DNA Hybridization. -- J.C. Wilbur and M.S. Urdea, Quantitation of Viral Nucleic Acids Using Branched DNA (bDNA) Signal Amplification. -- I. Bronstein and C.E.M. Olesen, Chemiluminescence Methods. -- B.J. McCreedy, Polymerase Chain Reaction. -- F. Ferre, P. Pezzoli, E. Buxton, C. Duffy, A. Marchese, and A. Diagle, Quantitation of RNA Targets Using the Polymerase Chain Reaction. -- J.B. Mahony and M.A. Chernesky, Multiplex Polymerase Chain Reaction (M-PCR). -- G.J. Nuovo, PCR in Situ Hybridization. -- R. Sooknanan, B. van Gemen, and L.T. Malek, Nucleic Acid Sequence Based Amplification (NASBA(tm)). -- S.S. Ghosh, E. Fahy, and T.R. Gingeras, The Self-Sustained Sequence Replication (3SR) Reaction and its Application in Clinical Diagnostics and Molecular Biology. -- J.D. Burczak, S. Ching, H.-Y. Hu, and H.H. Lee, Ligase Chain Reaction (LCR) for the Detection of Infectious Agents. -- G.T. Walker, et al., A Chemiluminescent DNA Probe Test Based upon Strand Displacement Amplification (SDA). -- D.M. Schuster, M.S. Berninger, and A. Rashtchian, Ligation Activated Transcription Amplification: Amplification and Detection of Human Papillomaviruses. -- Subject Index.

Includes index.

Nucleic acid detection methods / Danny L. Wiedbrauk and Ann M. Drevon -- Quality assurance in the molecular virology laboratory / Danny L. Wiedbrauk and Jay Stoerker -- Nucleic acid blotting techniques for virus detection / Daniel L. Stoler and Nelson L. Michael -- In situ hybridization / Jeanne Carr -- Antiviral susceptibility testing using DNA-DNA hybridization / Richard L. Hodinka -- Quantification of viral nucleic acids using branched DNA signal amplification / Judith C. Wilbur and Mickey S. Urdea -- Detection methods using chemiluminescence / Irena Bronstein and Corinne E.M. Olesen -- Detection of viral pathogens using PCR emplification / Bruce J. McCreedy -- Quantitation of RNA targets using the polymerase chain reaction / Francois Ferre [and others] -- Multiplex polymerase chain reaction / James B. Mahony and Max A. Chernesky -- PCR in situ hybridization / Gerard J. Nuovo -- Nucleic acid sequence-based amplification / Roy Sooknanan, Bob van Gemen, and Lawrence T. Malek -- The Self-sustained sequence replication reaction and its application in clinical diagnostics and molecular biology / Soumitra S. Ghosh, Eoin Fahy, and Thomas R. Gingeras -- Ligase chain reaction for the detection of infectious agents / John D. Burczak [and others] -- A Chemiluminescent DNA probe test based on strand displacement amplification / G.T. Walker [and others]-- Ligation-activated transcription amplification: amplification and detection of human papillomaviruses / David M. Schuster, Mark S. Berninger, and Ayoub Rashtchian.

Includes bibliographical references and index.

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