Protein phosphorylation : a practical approach / edited by D.G. Hardie.

Includes bibliographical references and index.

Print version record.

English.

Study of protein phosphorylation in intact cells / Timothy A.J. Haystead -- Analysis of signal transduction pathways using protein kinase inhibitors and activators / Yasuhito Naito -- Analysis of signal transduction pathways using protein-serine/threonine phosphatase inhibitors / D. Grahame Hardie -- Analysis of signal transduction pathways using molecular biological manipulations / Calum Sutherland -- Phosphopeptide mapping and phosphoamino acid analysis on cellulose thin-layer plates / Peter van der Geer -- Phosphorylation site analysis by mass spectrometry / Ken I. Mitchelhill -- Assay and purification of protein (serine/threonine) phosphatases / Carol Mackintosh -- Identification of substrates for protein-tyrosine phosphates / Andrew J. Garton -- Assay and purification of protein-serine/threonine kinases / D. Grahame Hardie -- Assay of protein kinases and phosphatases using specific peptide substrates / Maria Ruzzene -- Cloning and expression of cDNAs encoding protein kinase subunits / David Carling -- Interaction cloning of protein kinase partners / Julie M. Stone -- Identifying protein kinase substrates by expression screening with solid-phase phosphorylation / Rikiro Fukunaga -- Analysis of protein kinase interactions using the two-hybrid method / Rong-Jiang -- Analysis of protein kinase interactions using biomolecular interaction analysis / Friedrich -- Analysis of protein kinase specificity using oriented peptide libraries / Zhou Songyang -- Crystallization of protein kinases and phosphatases / Bostijan Kobe.

Reversible phosphorylation is one of the major mechanisms of controlling protein activity in all eukaryotic cells. This new edition of Protein Phosphorylation: A Practical Approach provides a comprehensive description of current methods used to study protein phosphorylation and the kinases and phosphatases which catalyse it. It includes protocols for studying phosphorylation in intact cells; analysis of signal transduction pathways, kinase specificity, and kinase interactions; assay and purification of kinases and phosphatases; and identification of substrates. Also covered are cloning and expression protocols and advice on the crystallization of kinases and phosphatases. Protein Phosphorylation: A Practical Approach 2e will therefore be of great value to any researcher investigating aspects of reversible protein phosphrylation.

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