In vitro fertilization / Kay Elder, Brian Dale.

On previous ed. Brian Dale appears first.

Includes bibliographical references and index.

Print version record.

This comprehensively updated and expanded second edition builds on its successful and popular predecessor, retaining the practical features which made the first edition such an essential guide to IVF. The edition describes additions to the range of ART clinical treatments, including the use of testicular and epididymal sperm, blastocyst stage transfer, and new perspectives in cryobiology and cryopreservation techniques. By incorporating laboratory techniques and protocols, with an even greater emphasis on quality control, it provides an indispensable and practical account. The introductory chapters covering the scientific background that underpins effective laboratory practice have been substantially expanded, derived from research in mammalian systems into the molecular biology of oogenesis, oocyte maturation, and early embryo metabolism. This second edition distils a wealth of practical and scientific detail for the benefit of all IVF practitioners.

Cover -- Half-title -- Title -- Copyright -- Dedication -- Contents -- Preface -- Acknowledgments -- 1 Introduction -- Further reading -- 2 Producing gametes -- Oocyte growth -- Follicle development -- Storing information -- The regional organization of the oocyte: polarization -- Oogenesis in the human -- Meiotic arrest and resumption of meiosis -- Resumption of meiosis -- Spermatogenesis in mammals -- Further reading -- 3 Sperm-oocyte interaction -- The acrosome and the vitelline coat -- Sperm-oocyte fusion -- Activation of the spermatozoon -- Motility -- Chemotaxis -- Capacitation.

Acrosome reaction -- Sperm-oocyte interaction in mammals -- Capacitation in mammals -- Acrosome reaction -- Sperm-oocyte fusion -- Oocyte activation -- Releasing meiotic arrest -- 1. The G-protein hypothesis -- 2. The soluble sperm factor hypothesis -- Intracellular calcium release -- Calcium and the cell cycle proteins -- The cortical reaction -- The cortical reaction in mammals -- The role of cortical reorganization -- Fusion, centrosomes and pronuclei -- Centrosomes -- Formation of pronuclei -- Pronucleus formation in mammals -- Syngamy -- 'The sun in the egg' -- Further reading.

4 First stages of development -- Activation of the zygote genome -- Imprinting -- Compaction -- Causes of embryo arrest -- Paternal factors -- Metabolic requirements of the early mammalian embryo in vitro Yves Ménézo -- Cleavage patterns -- Cytoplasmic segregation and the formation of cell lines -- Further reading -- 5 Endocrine control of reproduction -- Further reading -- 6 Assisted reproductive technology in farm animals -- Artificial insemination -- Bovine IVF -- (with thanks to R. Brittain) -- Maturation (IVM) -- Fertilization (IVF) -- Embryo culture (IVC) -- IVF during pregnancy.

Results -- The large calf syndrome -- Pregnancy results -- Sex distribution of in vitro produced calves -- Gender selection -- Microsurgery in mammalian embryos -- Further reading -- 7 The clinical in vitro fertilization laboratory -- Introduction -- Setting up a laboratory: equipment and facilities -- Ambient air quality -- Laboratory space layout -- Incubators -- Water quality -- Supplies -- Daily cleaning routine -- Washing procedures -- Pipettes -- Non-disposable items (handle with non-powered gloves, rinsed in purified water) -- Tissue culture media -- Serum -- Growth factors.

Follicular flushing -- Quality control procedures -- Suggested useful routine QC procedures include the following: -- 1. Sperm survival test -- 2. Culture of surplus oocytes -- 3. Multipronucleate embryo culture -- 4. Culture of 'spare' embryos -- Tissue culture systems -- Oil preparation -- Serum supplements -- Preparation of maternal serum -- Basic equipment required for the IVF laboratory -- Embryology -- Details of IVF media can be obtained from the following manufacturing companies: -- Further reading -- 8 Semen analysis and preparation for assisted reproductive techniques.

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