TY  - GEN
AU  - Ameur,Adam
AU  - Hestand,Matthew S.
TI  - Advances in Single Molecule, Real-Time (SMRT) Sequencing
SN  - books978-3-03921-701-4
PY  - 2019///
PB  - MDPI - Multidisciplinary Digital Publishing Institute
KW  - n/a
KW  - Cladobotryum protrusum
KW  - allele-specific analysis
KW  - low-input DNA
KW  - full length RNAseq
KW  - de novo genome assembly
KW  - de novo assembly
KW  - human reference genome
KW  - Tricoplusia ni
KW  - PacBio single molecule real-time sequencing
KW  - secondary metabolite
KW  - protein isoforms
KW  - bone marrow cell subpopulations
KW  - DNA methylation
KW  - mycoparasite
KW  - human whole-genome sequencing
KW  - GRCh38
KW  - SMRT sequencing
KW  - cytochrome P450 enzyme (CYP)
KW  - mRNA isoforms
KW  - next generation sequencing
KW  - cobweb disease
KW  - Swedish population
KW  - mosquito
KW  - long-read SMRT sequencing
KW  - whole genome sequencing
KW  - terpenoid
KW  - insect genome
KW  - optical mapping
KW  - Gloeostereum incarnatum
KW  - population sequencing
KW  - statistical methods
KW  - gene expression
KW  - single molecule real-time sequencing
KW  - PacBio
N1  - Open Access
N2  - PacBio's single-molecule real-time (SMRT) sequencing technology offers important advantages over the short-read DNA sequencing technologies that currently dominate the market. This includes exceptionally long read lengths (20 kb or more), unparalleled consensus accuracy, and the ability to sequence native, non-amplified DNA molecules. From fungi to insects to humans, long reads are now used to create highly accurate reference genomes by de novo assembly of genomic DNA and to obtain a comprehensive view of transcriptomes through the sequencing of full-length cDNAs. Besides reducing biases, sequencing native DNA also permits the direct measurement of DNA base modifications. Therefore, SMRT sequencing has become an attractive technology in many fields, such as agriculture, basic science, and medical research. The boundaries of SMRT sequencing are continuously being pushed by developments in bioinformatics and sample preparation. This book contains a collection of articles showcasing the latest developments and the breadth of applications enabled by SMRT sequencing technology
UR  - https://mdpi.com/books/pdfview/book/1798
UR  - https://directory.doabooks.org/handle/20.500.12854/40355
ER  -