TY  - BOOK
AU  - Fernandez,Joseph M.
AU  - Hoeffler,James P.
TI  - Gene expression systems: using nature for the art of expression 
SN  - 9780122538407
AV  - QH450 .G4626 1999eb
U1  - 572.8/65 22
PY  - 1999///
CY  - San Diego
PB  - Academic Press
KW  - Gene expression
KW  - Prokaryotes
KW  - Genetics
KW  - Insects
KW  - Transgenic animals
KW  - Eukaryotic cells
KW  - Prokaryotic Cells
KW  - Gene Expression Regulation
KW  - Gene Expression
KW  - Animals, Genetically Modified
KW  - genetics
KW  - Eukaryotic Cells
KW  - SCIENCE
KW  - Life Sciences
KW  - Genetics & Genomics
KW  - bisacsh
KW  - fast
KW  - Genexpressie
KW  - gtt
KW  - Genregulatie
KW  - Expression génique
KW  - ram
KW  - Procaryotes
KW  - Génétique
KW  - Insectes
KW  - Animaux transgéniques
KW  - Cellules eucaryotes
KW  - Electronic books
N1  - Includes bibliographical references and index; Contributors. -- Introduction: The Art of Expression. -- Purpose of This Book. -- Selecting a Suitable Expression System: -- Considerations. -- Genomics and the Future of Protein Expression Systems, J.M. Fernandez and J.P. Hoeffler. -- Prokaryotic Expression Systems: -- Gene Expression Systems Based on Bacteriophage T7 RNA Polymearse, R. Durbin. -- Expression Vectors Employing the trc Promoter, J. Brosius. -- Bacillus Expression: A Gram Positive Model, E. Ferrari and B. Miller. -- araB Expression System in Escherichia coli, M. Better. -- Eukaryotic Expression Systems: -- Adenoviral Vectors for Protein Expression, D.J. von Seggern and G.R. Nemerow. -- Expression in the Methalotrophic Yeast Pichia pastoris, J.M. Cregg. -- Recombinant Protein Expression in Pichia methanolica, C.K. Raymond. -- Cytomegalovirus Promoter for Expression in Mammalian Cells, M.F. Stinski. -- Inducible Mammalian Expression Systems, M. Russell. -- Protein Expression in Mammalian Cells Using Sindbis Virus, R.P. Bennett. -- Expression in Insect Systems: -- Drosophila S2 System for Heterologous Gene Expression, R.B. Kirkpatrick and A. Shatzman. -- Baculovirus Expression Vector System, M. Galleno and A.J. Sick. -- Transgenic Expression: -- Recombinant Protein Expression in Transgenic Mice, R. Abbud and J.H. Nilson. -- Expression of Recombinant Proteins in the Milk of Transgenic Animals, H.M. Meade, Y. Echelard, C.A. Ziomek, M.W. Young, M. Harvey, E.S. Cole, S. Groet, T.E. Smith, and J.M. Curling. -- Recombinant Protein Expression in Plants, A.E. Voloudakis, Y. Yin, and R.N. Beachy. -- Index; Gene expression systems based on bacteriophage T7 RNA polymerase -- Expression vectors employing the trc promoter -- Bacillus expression : a gram-positive model -- araB expression system in Escherichia coli -- Adenoviral vectors for protein expression -- Expression in the methylotrophic yeast Pichia pastoris -- Recombinant protein expression in Pichia methanolica -- Cytomegalovirus promoter for expression in mammalian cells -- Inducible mammalian expression systems -- Protein expression in mammalian cells using Sindbis virus -- Drosophila S2 system for heterologous gene expression -- Baculovirus expression vector system -- Recombinant protein expression in transgenic mice -- Expression of recombinant proteins in the milk of transgenic animals -- Recombinant protein expression in plants; Electronic reproduction; [Place of publication not identified]; HathiTrust Digital Library; 2010
N2  - Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression. Key Features * Provides detailed information on expression systems * Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs * Includes timelines and examples * Compares pros and cons of each method
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