An introduction to metabolic and cellular engineering / S. Cortassa [and others].

Includes bibliographical references and index.

Print version record.

Preface; Contents; List of Abbreviations; Introduction; Introductory Outlines; Metabolic and Cellular Engineering in the Context of Bioprocess Engineering; Tools for Metabolic and Cellular Engineering; Engineering Cells for Specific Biotransformations; Metabolic Areas that Have Been Subjected to MCE; From DNA Sequence to Biological Function; Temporal and Spatial Scaling in Cellular Processes; Scaling in Microbial and Biochemical Systems; Views of the Cell; Black and Grey Boxes: Levels of Description of Metabolic Behavior in Microorganisms; Transduction and Intracellular Signalling.

Self-organized Emergent PhenomenaHomeodynamics and Coherence; Matter and Energy Balances; Mass Balance; General Formulation of Mass Balance; Integral and Differential Mass Balances; Growth Stoichiometry and Product Formation; Biomass and Product Yields; Electron Balance; Theoretical Oxygen Demand; Opening the ""Black Box"". Mass Balance as the Basis of Metabolic Flux Analysis; Energy Balance; Forms of Energy and Enthalpy; Calorimetric Studies of Energy Metabolism; Heat of Combustion; An Energetic View of Microbial Metabolism; Cell Growth and Metabolite Production. Basic Concepts.

Microbial Growth under Steady and Balanced ConditionsMicrobial Energetics under Steady State Conditions; Growth Kinetics under Steady State Conditions; The Dilution Rate; The Dilution Rate and Biomass Concentration; The Dilution Rate and the Growth-limiting Substrate Concentration; Biomass and Growth-limiting Substrate Concentration at the Steady State; Growth as a Balance of Fluxes; The Flux Coordination Hypothesis; Toward a Rational Design of Cells; Redirecting Central Metabolic Pathways under Kinetic or Thermodynamic Control.

Thermodynamic or Kinetic Control of Flux under Steady State ConditionsKinetic and Thermodynamic Limitations in Microbial Systems. Case Studies; Saccharomyces cerevisiae; Escherichia coli; Increasing Carbon Flow to Aromatic Biosynthesis in Escherichia coli; Methods of Quantitation of Cellular Processes Performance -- Stoichiometry of Growth: The Equivalence between Biochemical Stoichiometries and Physiological Parameters; A General Formalism for Metabolic Flux Analysis; A Comparison between Different Methods of MFA; MFA Applied to Prokaryotic and Lower Eukaryotic Organisms.

MFA as Applied to Studying the Performance of Mammalian Cells in CultureMetabolic Fluxes during Balanced and Steady State Growth; Bioenergetic and Physiological Studies in Batch and Continuous Cultures. Genetic or Epigenetic Redirection of Metabolic Flux; Introduction of Heterologous Metabolic Pathways; Metabolic Engineering of Lactic Acid Bacteria for Optimising Essential Flavor Compounds Production; Metabolic Control Analysis; Summation and connectivity theorems; Control and Regulation; The Control of Metabolites Concentration.

Metabolic and cellular engineering, as presented in this book, is a powerful alliance of two technologies: genetics â€" molecular biology and fermentation technology. Both are driven by continuous refinement of the basic understanding of metabolism, physiology and cellular biology (growth, division, differentiation), as well as the development of new mathematical modeling techniques. The authorsâ€TM approach is original in that it integrates several disciplines into a coordinated scheme, i.e. microbial physiology and bioenergetics, thermodynamics and enzyme kinetics, biomathematics and biochemi.

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