Molecular protocols in transfusion medicine / Gregory A. Denomme, Maria Rios, Marion E. Reid.

By:

Denomme, Gregory A

Contributor(s):

Material type: Text

TextPublication details: San Diego, CA : Academic Press, 2000.Description: 1 online resource (x, 186 pages) : illustrationsContent type:

text

Media type:

computer

Carrier type:

online resource

ISBN:

9780122093708
0122093704
9781435608092
1435608097
0080536905
9780080536903
128103276X
9781281032768

Subject(s): Genre/Form:

Additional physical formats: Print version:: Molecular protocols in transfusion medicine.DDC classification:

612.1/1825 22

LOC classification:

QP98 .D46 2000eb

NLM classification:

2000 H-750
QY 415

Online resources:

Click here to access online

Contents:

Front Cover; Molecular Protocols in Transfusion Medicine; Copyright Page; Contents; Preface; List of Abbreviations; Part 1: Introduction; Part 2: General Considerations; Part 3: Protocols; Part 4: Red Cell Blood Groups; Part 5: Platelet Blood Groups; Part 6: Neutrophil Blood Groups; Part 7: Appendices; Index; Useful Tables.

Summary: This essential methods manual for immunohematologists (or hematologists and immunohematologists) provides information on genes that encode antigens on red blood cells, platelets and neutrophils. The book begins by covering general concepts in molecular biology and specific protocols such as DNA preparation, PCR-RFLP and allele-specific PCR. Information on the erythrocyte, platelet and neutrophil antigen systems and the molecular basis of polymorphisms are presented clearly in a gene facts sheet format. Database accession numbers and useful adjuncts such as Request forms, worksheets for PCR/enzyme digests also serve to benefit the user. The information is clearly presented and easily accessible and is complemented by the excellent diagrams and tabular material. This book is invaluable for both new and experienced researchers in the field and other related disciplines. Key Features * Essential for hematologists and those involoved in tissue typing and the study of human genetic polymorphisms * Presents clearly and concisely the information on a particular variant and the technique used to detect it * Organized by antigen and provides sequences of polymorphisms and primers * Details the general concepts and critical information on genes, their products, and sources of relevant nucleic acids * Includes protocols that allow investigators to set up assays with minimal effort (protocols include primers, reagents, reaction conditions, sizes of amplified products, restriction fragment digests, and the relevant safety information) * Provides information that helps interpret results in clinical settings * Contains additional sources of information (e.g., key references, web site addresses, glossary, Database accession numbers, request forms, and worksheets for PCR/enzyme digests).

Item type:

Tags from this library: No tags from this library for this title. Log in to add tags.

Average rating: 0.0 (0 votes)

Holdings
Item type	Home library	Collection	Call number	Materials specified	Status	Date due	Barcode
Electronic-Books	OPJGU Sonepat- Campus	E-Books EBSCO			Available

This essential methods manual for immunohematologists (or hematologists and immunohematologists) provides information on genes that encode antigens on red blood cells, platelets and neutrophils. The book begins by covering general concepts in molecular biology and specific protocols such as DNA preparation, PCR-RFLP and allele-specific PCR. Information on the erythrocyte, platelet and neutrophil antigen systems and the molecular basis of polymorphisms are presented clearly in a gene facts sheet format. Database accession numbers and useful adjuncts such as Request forms, worksheets for PCR/enzyme digests also serve to benefit the user. The information is clearly presented and easily accessible and is complemented by the excellent diagrams and tabular material. This book is invaluable for both new and experienced researchers in the field and other related disciplines. Key Features * Essential for hematologists and those involoved in tissue typing and the study of human genetic polymorphisms * Presents clearly and concisely the information on a particular variant and the technique used to detect it * Organized by antigen and provides sequences of polymorphisms and primers * Details the general concepts and critical information on genes, their products, and sources of relevant nucleic acids * Includes protocols that allow investigators to set up assays with minimal effort (protocols include primers, reagents, reaction conditions, sizes of amplified products, restriction fragment digests, and the relevant safety information) * Provides information that helps interpret results in clinical settings * Contains additional sources of information (e.g., key references, web site addresses, glossary, Database accession numbers, request forms, and worksheets for PCR/enzyme digests).