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Biological Specimen Preparation for Transmission Electron Microscopy.

By: Contributor(s): Material type: TextTextSeries: Princeton legacy libraryPublication details: Princeton : Princeton University Press, 2014.Description: 1 online resource (349 pages)Content type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 9781400865024
  • 1400865026
Subject(s): Genre/Form: Additional physical formats: Print version:: Biological Specimen Preparation for Transmission Electron Microscopy.DDC classification:
  • 570.2827
LOC classification:
  • QH239
Online resources:
Contents:
Cover; 1 An Introduction to Fixation and Embedding Procedures and Their Safe Use in the Laboratory; 2 Fixatives; 3 Fixation Methods; 4 Dehydration Methods; 5 Embedding Methods; 6 Embedding in Epoxy Resins; 7 Embedding in Acrylic Resins; 8 The Lowicryl Resins and Embedding at Low Temperatures; 9 Other Embedding Media; 10 Processing Schedules; Appendix: List of Suppliers; Subject Index.
Summary: This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen. The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify est.
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Print version record.

Cover; 1 An Introduction to Fixation and Embedding Procedures and Their Safe Use in the Laboratory; 2 Fixatives; 3 Fixation Methods; 4 Dehydration Methods; 5 Embedding Methods; 6 Embedding in Epoxy Resins; 7 Embedding in Acrylic Resins; 8 The Lowicryl Resins and Embedding at Low Temperatures; 9 Other Embedding Media; 10 Processing Schedules; Appendix: List of Suppliers; Subject Index.

This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen. The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify est.

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