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OCoLC |
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20220712224334.0 |
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98016653 |
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SFB |
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eng |
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pn |
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SFB |
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OCLCQ |
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| International Standard Book Number |
1429401311 |
| Qualifying information |
(electronic bk.) |
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9781429401319 |
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(electronic bk.) |
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0195119266 |
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9780195119268 |
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1280529784 |
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9781280529788 |
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9786610529780 |
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6610529787 |
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0198028016 |
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9780198028017 |
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AU@ |
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000051585313 |
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802633617 |
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NZ1 |
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12438118 |
| 035 ## - SYSTEM CONTROL NUMBER |
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(OCoLC)243613675 |
| Canceled/invalid control number |
(OCoLC)71523301 |
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(OCoLC)144217398 |
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(OCoLC)473438365 |
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(OCoLC)560383056 |
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(OCoLC)647576049 |
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(OCoLC)821693437 |
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(OCoLC)888812036 |
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(OCoLC)1037782117 |
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(OCoLC)1110358060 |
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(OCoLC)1153472876 |
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(OCoLC)1162225907 |
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(OCoLC)1228537431 |
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(OCoLC)1241871224 |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER |
|---|
| Classification number |
QP606.D46 |
| Item number |
H37 1998eb |
| 060 #4 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER |
|---|
| Classification number |
1999 D-687 |
| 060 #4 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER |
|---|
| Classification number |
QH 450.3 |
| Item number |
H313L 1998 |
| 072 #7 - SUBJECT CATEGORY CODE |
|---|
| Subject category code |
SCI |
| Subject category code subdivision |
049000 |
| Source |
bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE |
|---|
| Subject category code |
PDN |
| Source |
bicssc |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
|---|
| Classification number |
572.8 |
| Edition number |
22 |
| 084 ## - OTHER CLASSIFICATION NUMBER |
|---|
| Classification number |
WC 4460 |
| Source of number |
rvk |
| 084 ## - OTHER CLASSIFICATION NUMBER |
|---|
| Classification number |
BIO 220f |
| Source of number |
stub |
| 084 ## - OTHER CLASSIFICATION NUMBER |
|---|
| Classification number |
BIO 180f |
| Source of number |
stub |
| 084 ## - OTHER CLASSIFICATION NUMBER |
|---|
| Classification number |
CHE 860f |
| Source of number |
stub |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
MAIN |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Harris, Eva, |
| Dates associated with a name |
1965- |
| 9 (RLIN) |
164352 |
| 245 12 - TITLE STATEMENT |
|---|
| Title |
A low-cost approach to PCR : |
| Remainder of title |
appropriate transfer of biomolecular techniques / |
| Statement of responsibility, etc |
Eva Harris ; edited by Nazreen Kadir. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc |
New York : |
| Name of publisher, distributor, etc |
Oxford University Press, |
| Date of publication, distribution, etc |
1998. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
1 online resource (xxi, 304 pages) : |
| Other physical details |
illustrations |
| 336 ## - |
|---|
| -- |
text |
| -- |
txt |
| -- |
rdacontent |
| 337 ## - |
|---|
| -- |
computer |
| -- |
c |
| -- |
rdamedia |
| 338 ## - |
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| -- |
online resource |
| -- |
cr |
| -- |
rdacarrier |
| 347 ## - |
|---|
| -- |
data file |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
|---|
| Bibliography, etc |
Includes bibliographical references and index. |
| 505 0# - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
1 Introduction -- 2 PCR Technology -- 2.1. Description of the Technique -- 2.1.1. Historical Overview -- 2.1.2. The Molecular Basis of PCR -- 2.1.3. Advantages and Disadvantages -- 2.2. Technical Details -- 2.2.1. Components of the PCR Mixture -- 2.2.2. Thermal Cycling Parameters -- 2.2.3. Optimizing -- 2.2.4. Potential Problems -- 2.2.5. Detection of Products -- 2.3. Frequently-Used PCR-Based Techniques -- 3 Principles of Sustainable Technology Transfer -- 3.1. A Low-Cost Methodology -- 3.1.1. Appropriate Technology -- 3.1.2. Alternative Techniques -- 3.1.3. Simplification of Protocols -- 3.1.4. In-House Preparation of Reagents -- 3.1.5. Recycling -- 3.1.6. Donated Materials -- 3.2. Knowledge-Based Participatory Transfer Process -- 3.3. Appropriate Application -- 3.3.1. General Considerations -- 3.3.2. An Evaluation Framework -- 3.3.3. Assessment Criteria for PCR -- 3.3.4. Case-by-Case Evaluation of PCR Applied to Infectious Diseases -- 3.4. Intra-Reginal Cooperation. |
| 505 0# - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
4 Case Study: The AMB/ATT Program -- 4.1. Introduction -- 4.2. Program Description -- 4.2.1. Objectives -- 4.2.2. Format -- 4.3. Program Structure -- 4.3.1. Phase -- 4.3.2. Phase -- 4.3.3. Phase -- 4.4. Program Development -- 4.5. Examples of Projects -- 4.6. International Courses -- II Selected Protocols -- 5.1. PCR Protocols -- 5.1.1. Overview -- 5.1.2. Equipment, Materials, Control, and Procedures Common to All PCR Protocols -- 5.1.3. Dengue Virus -- 5.1.4. New World Leishmania -- 5.1.5. Mycobactrium tuberculosis -- 5.1.6. Plasmodium falciparum and Plasmodium vivax -- 5.1.7. Vibrio cholerae -- 5.1.8. Diarrheagenic E. coli and Shigella -- 5.1.9. Chlamydia trachomatis and Neisseria gonorrhoeae -- 5.1.10. Leptospira -- 5.1.11. Trypanosoma cruzi -- 5.2. Nonradioactive DNA Probes: V. cholerae Colony Blot -- 5.2.1. Labeling the Probe Using PCR -- 5.2.2. Preparation of the Colony Blot -- 5.2.3. Hybridization -- 5.2.4. Visualization -- 6 Rapid Cloning of PCR Products -- 6.1. Primer Design. |
| 505 0# - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
6.2. Preparation of PCR Products for Cloning -- 6.2.1. Digestion of Vector and PCR Products -- 6.2.2. Preparative Agarose Gel -- 6.2.3. Purification of the Excised DNA Fragments Using Silica Particles -- 6.3. Ligation -- 6.3.1. Checking DNA Fragment Concentration by Agarose Gel Electrophoresis -- 6.3.2. Ligation Reaction -- 6.4. Preparation of Competent Cells and Transformation -- 6.4.1. Preparation of Competent Cells -- 6.4.2. Transformation -- 6.5. Checking Clones by PCR -- 6.6. Plasmid Purification -- 6.7. Analysis of Clones by Restriction Enzyme Digestion -- 6.8. Agarose Gel Electrophoresis -- III Appendix -- A. Construction of Laboratory Equipment -- B. In-House Preparation of Reagents -- B.1 Useful Formulas -- B.2 Solutions -- B.3 Preparation of Selected Reagents -- B.4 DNA Size Markers -- C. Inventory for a PCR Laboratory -- D. Good Laboratory Practice -- D.1 General Tips -- D.2 Calibration of Adjustable Pipettors -- E. Prevention of Cross-Contamination -- F. PCR Troubleshooting Guide and Flow-Chart -- G. Workshop Organization and Teaching Tips -- H. Sample Charts and Worksheets -- I. Useful World Wide Web Sites. |
| 588 0# - |
|---|
| -- |
Print version record. |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc |
The polymerase chain reaction (PCR) is a technique used to replicate specific pieces of DNA millions of times, which permits the detection and analysis of minute amounts of nucleic acids. Since its introduction in the late 1980s, this technique has been applied not only in molecular biology research but also in fields as diverse as anthropology, phylogeny, and forensics. However, despite the large impact of PCR, many of its applications remain within the confines of research and the academic environment. Now, in A Low-Cost Approach to PCR: Appropriate Transfer of Biomolecular Techniques, Dr. Eva Harris makes this elegantly simple technique more accessible to researchers, physicians, and laboratory workers throughout the world. She provides a description of the theoretical basis of the technique, the practical details of the method, and the philosophy behind the technology transfer program that she developed over the last ten years. The book serves as a guide for potential users in developing countries and for scientists in developed countries who may wish to work abroad.; In addition, the low-cost approach outlined in this book can be useful for high school, undergraduate, or continuing education programs in the United States. While the specific applications of PCR outlined in the book are immediately useful to the study of infectious diseases, the approach presented can be generalized to a number of other technologies and situations. The book will help laboratories in many areas of the world generate information on site for use by physicians, epidemiologists, public health workers, and health policy professionals to develop new strategies for disease control. |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
English. |
| 590 ## - LOCAL NOTE (RLIN) |
|---|
| Local note |
eBooks on EBSCOhost |
| Provenance (VM) [OBSOLETE] |
EBSCO eBook Subscription Academic Collection - Worldwide |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Molecular biology |
| General subdivision |
Technology transfer. |
| 9 (RLIN) |
164353 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Polymerase chain reaction |
| Form subdivision |
Laboratory manuals. |
| 9 (RLIN) |
164354 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Polymerase chain reaction. |
| 9 (RLIN) |
171369 |
| 650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Polymerase Chain Reaction |
| 9 (RLIN) |
171369 |
| 650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Molecular Biology |
| 9 (RLIN) |
71924 |
| 650 #6 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Réaction en chaîne de la polymérase |
| Form subdivision |
Manuels de laboratoire. |
| 9 (RLIN) |
1342652 |
| 650 #6 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Réaction en chaîne de la polymérase. |
| 9 (RLIN) |
1330828 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
SCIENCE |
| General subdivision |
Life Sciences |
| -- |
Molecular Biology. |
| Source of heading or term |
bisacsh |
| 9 (RLIN) |
878288 |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Biologia molecular. |
| Source of heading or term |
dcs |
| 9 (RLIN) |
1002866 |
| 650 27 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Reaccion en cadena de la polimerasa. |
| Source of heading or term |
dcs |
| 9 (RLIN) |
1407574 |
| 650 27 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Transferencia de tecnologia. |
| Source of heading or term |
dcs |
| 9 (RLIN) |
1407575 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Molecular biology |
| General subdivision |
Technology transfer. |
| Source of heading or term |
fast |
| -- |
(OCoLC)fst01024759 |
| 9 (RLIN) |
164353 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Polymerase chain reaction. |
| Source of heading or term |
fast |
| -- |
(OCoLC)fst01070553 |
| 9 (RLIN) |
171369 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Methode |
| Source of heading or term |
gnd |
| 9 (RLIN) |
868182 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Polymerase-Kettenreaktion |
| Source of heading or term |
gnd |
| 9 (RLIN) |
1407576 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Vereinfachung |
| Source of heading or term |
gnd |
| 9 (RLIN) |
1407577 |
| 655 #2 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Laboratory Manual |
| 9 (RLIN) |
868475 |
| 655 #4 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Electronic books. |
| 655 #7 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Laboratory manuals. |
| Source of term |
fast |
| -- |
(OCoLC)fst01920776 |
| 9 (RLIN) |
849443 |
| 655 #7 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Laboratory manuals. |
| Source of term |
lcgft |
| 9 (RLIN) |
849443 |
| 655 #7 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Manuels de laboratoire. |
| Source of term |
rvmgf |
| 9 (RLIN) |
868476 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Kadir, Nazreen. |
| 9 (RLIN) |
164355 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY |
|---|
| Display text |
Print version: |
| Main entry heading |
Harris, Eva, 1965- |
| Title |
Low-cost approach to PCR. |
| Place, publisher, and date of publication |
New York : Oxford University Press, 1998 |
| Record control number |
(DLC) 98016653 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
<a href="https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=169030">https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=169030</a> |
| 938 ## - |
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ebrary |
| -- |
EBRY |
| -- |
ebr10142260 |
| 938 ## - |
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| -- |
EBSCOhost |
| -- |
EBSC |
| -- |
169030 |
| 938 ## - |
|---|
| -- |
ProQuest MyiLibrary Digital eBook Collection |
| -- |
IDEB |
| -- |
52978 |
| 938 ## - |
|---|
| -- |
Internet Archive |
| -- |
INAR |
| -- |
lowcostapproacht0000harr |
| 938 ## - |
|---|
| -- |
YBP Library Services |
| -- |
YANK |
| -- |
2470986 |
| 938 ## - |
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| -- |
YBP Library Services |
| -- |
YANK |
| -- |
2875768 |
| 994 ## - |
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| -- |
92 |
| -- |
INOPJ |
