Gene expression systems : using nature for the art of expression /

Gene expression systems : using nature for the art of expression / edited by Joseph M. Fernandez and James P. Hoeffler. - San Diego : Academic Press, ©1999. - 1 online resource (xii, 480 pages) : illustrations

Includes bibliographical references and index.

Contributors. -- Introduction: The Art of Expression. -- Purpose of This Book. -- Selecting a Suitable Expression System: -- Considerations. -- Genomics and the Future of Protein Expression Systems, J.M. Fernandez and J.P. Hoeffler. -- Prokaryotic Expression Systems: -- Gene Expression Systems Based on Bacteriophage T7 RNA Polymearse, R. Durbin. -- Expression Vectors Employing the trc Promoter, J. Brosius. -- Bacillus Expression: A Gram Positive Model, E. Ferrari and B. Miller. -- araB Expression System in Escherichia coli, M. Better. -- Eukaryotic Expression Systems: -- Adenoviral Vectors for Protein Expression, D.J. von Seggern and G.R. Nemerow. -- Expression in the Methalotrophic Yeast Pichia pastoris, J.M. Cregg. -- Recombinant Protein Expression in Pichia methanolica, C.K. Raymond. -- Cytomegalovirus Promoter for Expression in Mammalian Cells, M.F. Stinski. -- Inducible Mammalian Expression Systems, M. Russell. -- Protein Expression in Mammalian Cells Using Sindbis Virus, R.P. Bennett. -- Expression in Insect Systems: -- Drosophila S2 System for Heterologous Gene Expression, R.B. Kirkpatrick and A. Shatzman. -- Baculovirus Expression Vector System, M. Galleno and A.J. Sick. -- Transgenic Expression: -- Recombinant Protein Expression in Transgenic Mice, R. Abbud and J.H. Nilson. -- Expression of Recombinant Proteins in the Milk of Transgenic Animals, H.M. Meade, Y. Echelard, C.A. Ziomek, M.W. Young, M. Harvey, E.S. Cole, S. Groet, T.E. Smith, and J.M. Curling. -- Recombinant Protein Expression in Plants, A.E. Voloudakis, Y. Yin, and R.N. Beachy. -- Index. Gene expression systems based on bacteriophage T7 RNA polymerase -- Expression vectors employing the trc promoter -- Bacillus expression : a gram-positive model -- araB expression system in Escherichia coli -- Adenoviral vectors for protein expression -- Expression in the methylotrophic yeast Pichia pastoris -- Recombinant protein expression in Pichia methanolica -- Cytomegalovirus promoter for expression in mammalian cells -- Inducible mammalian expression systems -- Protein expression in mammalian cells using Sindbis virus -- Drosophila S2 system for heterologous gene expression -- Baculovirus expression vector system -- Recombinant protein expression in transgenic mice -- Expression of recombinant proteins in the milk of transgenic animals -- Recombinant protein expression in plants.

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Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression. Key Features * Provides detailed information on expression systems * Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs * Includes timelines and examples * Compares pros and cons of each method.


Electronic reproduction.
[Place of publication not identified] :
HathiTrust Digital Library,
2010.


Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
http://purl.oclc.org/DLF/benchrepro0212


English.

9780122538407 0122538404 9780080532356 (electronic bk.) 0080532357 (electronic bk.) 1281054186 9781281054180 9786611054182 6611054189

78073:78073 Elsevier Science & Technology http://www.sciencedirect.com

GB9926654 bnb


Gene expression.
Prokaryotes--Genetics.
Insects--Genetics.
Transgenic animals--Genetics.
Eukaryotic cells.
Prokaryotic Cells.
Gene Expression Regulation.
Gene Expression.
Animals, Genetically Modified--genetics.
Eukaryotic Cells.
SCIENCE--Life Sciences--Genetics & Genomics.
Eukaryotic cells.
Gene expression.
Insects--Genetics.
Prokaryotes--Genetics.
Genexpressie.
Genregulatie.
Expression génique.
Procaryotes--Génétique.
Insectes.
Animaux transgéniques.
Cellules eucaryotes.


Electronic books.

QH450 / .G4626 1999eb

572.8/65

QH450.G4627 / 1999

1999 A-932 QH 450

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