Scanning electron microscopy for the life sciences /
Schatten, Heide,
Scanning electron microscopy for the life sciences / Heide Schatten. - Cambridge ; New York : Cambridge University Press, 2013. - 1 online resource - Advances in microscopy and microanalysis . - Advances in microscopy and microanalysis. .
Includes index.
Includes bibliographical references and index.
1. The role of scanning electron microscopy in cell and molecular biology: SEM basics, past accomplishments, and new frontiers -- 2. Corrosion casting technique -- 3. Revealing the internal structure of cells in three dimensions with scanning electron microscopy -- 4. Mitochondrial continuous intracellular network-structures visualized with high-resolution field-emission scanning electron microscopy -- 5. Is the scanning mode the future of electron microscopy in cell biology? -- 6. High-resolution labeling for correlative microscopy -- 7. The use of SEM to explore viral structure and trafficking -- 8. High-resolution scanning electron microscopy of the nuclear surface in Herpes Simplex Virus 1 infected cells -- 9. Scanning electron microscopy of chromosomes: structural and analytical investigations -- 10. A method to visualize the microarchitecture of glycoprotein matrices with scanning electron microscopy -- 11. Scanning electron microscopy of cerebellar intrinsic circuits -- 12. Application of in vivo cryotechnique to living animal organs examined by scanning electron microscopy -- 13. SEM in dental research -- 14. SEM, teeth, and palaeoanthropology: the secret of ancient human diets.
Recent developments in scanning electron microscopy (SEM) have resulted in a wealth of new applications for cell and molecular biology, as well as related biological disciplines. It is now possible to analyze macromolecular complexes within their three-dimensional cellular microenvironment in near native states at high resolution and to identify specific molecules and their structural and molecular interactions. New approaches include cryo-SEM applications and environmental SEM (ESEM), staining techniques and processing applications combining embedding and resin-extraction for imaging with high resolution SEM, and advances in immuno-labeling. New developments include helium ion microscopy, automated block-face imaging combined with serial sectioning inside an SEM chamber, and Focused Ion Beam Milling (FIB) combined with block-face SEM. With chapters written by experts, this guide gives an overview of SEM and sample processing for SEM and highlights several advances in cell and molecular biology that greatly benefited from using conventional, cryo, immuno and high-resolution SEM.
English.
9781139839600 (electronic bk.) 1139839608 (electronic bk.) 9781139018173 (electronic bk.) 1139018175 (electronic bk.) 9781139841986 113984198X 9781139844345 (e-book) 1139844342 9781139844345 9781139840798 1139840797 1107232872 9781107232877 1139853430 9781139853439 1139845497 9781139845496
99952413566
412501 MIL
016102713 Uk
Biology--Methodology.
Scanning electron microscopy.
Biologie--Méthodologie.
Microscopie électronique à balayage.
NATURE--Reference.
SCIENCE--Life Sciences--Biology.
SCIENCE--Life Sciences--General.
Biology--Methodology.
Scanning electron microscopy.
Electronic books.
Electronic books.
Electronic books.
QH324.9.X2 / S33 2013eb
570.28/25
Scanning electron microscopy for the life sciences / Heide Schatten. - Cambridge ; New York : Cambridge University Press, 2013. - 1 online resource - Advances in microscopy and microanalysis . - Advances in microscopy and microanalysis. .
Includes index.
Includes bibliographical references and index.
1. The role of scanning electron microscopy in cell and molecular biology: SEM basics, past accomplishments, and new frontiers -- 2. Corrosion casting technique -- 3. Revealing the internal structure of cells in three dimensions with scanning electron microscopy -- 4. Mitochondrial continuous intracellular network-structures visualized with high-resolution field-emission scanning electron microscopy -- 5. Is the scanning mode the future of electron microscopy in cell biology? -- 6. High-resolution labeling for correlative microscopy -- 7. The use of SEM to explore viral structure and trafficking -- 8. High-resolution scanning electron microscopy of the nuclear surface in Herpes Simplex Virus 1 infected cells -- 9. Scanning electron microscopy of chromosomes: structural and analytical investigations -- 10. A method to visualize the microarchitecture of glycoprotein matrices with scanning electron microscopy -- 11. Scanning electron microscopy of cerebellar intrinsic circuits -- 12. Application of in vivo cryotechnique to living animal organs examined by scanning electron microscopy -- 13. SEM in dental research -- 14. SEM, teeth, and palaeoanthropology: the secret of ancient human diets.
Recent developments in scanning electron microscopy (SEM) have resulted in a wealth of new applications for cell and molecular biology, as well as related biological disciplines. It is now possible to analyze macromolecular complexes within their three-dimensional cellular microenvironment in near native states at high resolution and to identify specific molecules and their structural and molecular interactions. New approaches include cryo-SEM applications and environmental SEM (ESEM), staining techniques and processing applications combining embedding and resin-extraction for imaging with high resolution SEM, and advances in immuno-labeling. New developments include helium ion microscopy, automated block-face imaging combined with serial sectioning inside an SEM chamber, and Focused Ion Beam Milling (FIB) combined with block-face SEM. With chapters written by experts, this guide gives an overview of SEM and sample processing for SEM and highlights several advances in cell and molecular biology that greatly benefited from using conventional, cryo, immuno and high-resolution SEM.
English.
9781139839600 (electronic bk.) 1139839608 (electronic bk.) 9781139018173 (electronic bk.) 1139018175 (electronic bk.) 9781139841986 113984198X 9781139844345 (e-book) 1139844342 9781139844345 9781139840798 1139840797 1107232872 9781107232877 1139853430 9781139853439 1139845497 9781139845496
99952413566
412501 MIL
016102713 Uk
Biology--Methodology.
Scanning electron microscopy.
Biologie--Méthodologie.
Microscopie électronique à balayage.
NATURE--Reference.
SCIENCE--Life Sciences--Biology.
SCIENCE--Life Sciences--General.
Biology--Methodology.
Scanning electron microscopy.
Electronic books.
Electronic books.
Electronic books.
QH324.9.X2 / S33 2013eb
570.28/25